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Literature summary extracted from

  • Chacon-Verdu, M.D.; Campillo-Brocal, J.C.; Lucas-Elio, P.; Davidson, V.L.; Sanchez-Amat, A.
    Characterization of recombinant biosynthetic precursors of the cysteine tryptophylquinone cofactors of l-lysine-epsilon-oxidase and glycine oxidase from Marinomonas mediterranea (2015), Biochim. Biophys. Acta, 1854, 1123-1131 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.20 expressed in Escherichia coli Rosetta(DE3) cells Marinomonas mediterranea

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.20 W581A inactive Marinomonas mediterranea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.20 L-lysine + O2 + H2O Marinomonas mediterranea
-
 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
1.4.3.20 L-lysine + O2 + H2O Marinomonas mediterranea NBRC 103028
-
 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.20 Marinomonas mediterranea F2JXJ3
-
-
1.4.3.20 Marinomonas mediterranea NBRC 103028 F2JXJ3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.20 Ni-NTA resin column chromatography Marinomonas mediterranea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.20 L-lysine + O2 + H2O
-
 Marinomonas mediterranea (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
1.4.3.20 L-lysine + O2 + H2O
-
 Marinomonas mediterranea NBRC 103028 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.20 ? x * 85000, SDS-PAGE Marinomonas mediterranea
1.4.3.20 ? x * 83071, calculated from amino acid sequence Marinomonas mediterranea

Synonyms

EC Number Synonyms Comment Organism
1.4.3.20 L-lysine-epsilon-oxidase
-
 Marinomonas mediterranea
1.4.3.20 LodA
-
 Marinomonas mediterranea

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.20 cysteine tryptophylquinone
-
 Marinomonas mediterranea